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Description
To add Illumina sequencing adaptors and custom indices, a second-round PCR was performed in a 40 l volume containing 2 l of the first-round product, 1 l each of 10 M P5 and P7 custom index primers, 0.4 l of Phusion High-Fidelity DNA Polymerase (NEB, no
[DOI] [PMC free article] [PubMed] [Google Scholar] [32].Monneaux F, Muller S
"Detection and in vitro metabolism of AOD9604"
noted decreased GPX3 expression in HF induced by DCM and ICM [89]
