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Large continuous fluxes of NO are necessary to keep ribonucleotide reductase inhibited, which would occur only under major inflammatory conditions or in the neighborhood of an activated macrophage

PMID: 65 NagaiTBridenbaughEAGashevAA

Since PV3-SC8 has previously been shown to bind pocket factors such as GPP3 in the VP1 hydrophobic pocket 18 , we investigated in situ if GSH binding interferes with this stabilisation mechanism by incubating a molar excess of GPP3 (VLP:compound molar ratio of 1:300) followed by GSH at 10 mM to form a ternary complex (PV3-SC8 GPP3+GSH ) with the PV3-SC8 VLP expressed in yeast 16

[DOI] [PubMed] [Google Scholar] 267.Hamer HM, Jonkers D, Venema K, Vanhoutvin S, Troost FJ, Brummer RJ