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Tirilazad mesylate accumulates at the BBB and has limited penetration into the brain
(PubMed) Penney KL, Schumacher FR, Li H, et al

S., Halsted, J

3.5 Removal of GST affinity tag after protease cleavage in solution If the protein was digested in solution, the target protein can be purified further by re-chromatography on the glutathione Sepharose column to remove any un-cleaved fusion protein and the GST moiety
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