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The running buffer containing dPBS and 0.01% Tween 20 was 0.22-m sterile filtered and degassed for at least 3 h
M.PatelM.ShchepinovM
I.et al (2019)
Metal-chelate affinity precipitation was also used to separate and purify -PL, which afforded electrophoretic purity with recovery as high as 98.42% (Li et al., 2017a)
