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Description
Cells from a 50 mL overnight culture in LB medium were harvested by centrifugation (4,000 g, 10 min) and resuspended in 20 mL MOPS minimal medium ( 578 ~ 0.2) containing 50 g mL 1 ampicillin (pGEX-6P-1- cntAB - bcct ), 17 g mL 1 chloramphenicol (pACYCDuet-1- msadh - mdh ) and 500 M IPTG
10.1016/s0022-5347(05)68087-1 97 TelokenPKatzD

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