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To purify secreted proteins that are produced from eukaryotic cell cultures (such as insect cells, CHO cells, 293 cells etc.), a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column

0.05) between the good and poor freezer in the group II and there was no difference in the group I and III under study at 0 and 1 hr incubation period and at 2 hrs the group III and at 4 hrs group I has revealed significance difference (p<

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