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Metal toxicity can have acute or chronic manifestations

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4), in which hydrogen peroxide oxidizes the ferrous ion, producing a ferric radical which reacts with the xylenol orange dye (3,3-bis(N,N-bis(carboxymethyl)aminomethyl)- o -cresolsulfonephthalein) in a ~ 1:4 stoichiometric relationship to form a chromophore complex (Gupta 1973)

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