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To further study the dynamics of glucose fluxes in B cell subsets, we measured glucose levels in the culture medium of Gclc fl/fl and Gclc fl/fl Mb1-Cre + FoB after 5 h activation with anti-IgM, CD40 ligand and IL-4

Tarangelo et al, 2018)

HepG2, HT1080, and MEF cells were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (P/S) at 37 C with 5% CO 2

Based at least in part on the experimental results presented herein, a ratio of about 3:2 sodium: chloride (e.g., about 2.5:2, about 2.75:2, or about 3:2 sodium: chloride) may be important for maintaining membrane integrity and stability