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From these findings and the fact that glutathione is essential for lymphocyte proliferation and immune responses, , we hypothesized that abundant cystine/cysteine in the kidneys forms a unique metabolic microenvironment that supports ectopic lymphocyte proliferation, leading to the formation of TLS
Briefly, 24 h after transfection, cells were harvested and resuspended in PBS at a density of 4 10 6 cells/ml

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