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Description
DNA methylation-based classification of central nervous system tumours
Bioengineered 13 , 38773895 (2022)
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Each 20 mL PCR reaction contained 1 mL of 1/20-fold diluted cDNA, 1X Phusion GC buffer, 0.2 mM deoxynucleotide triphosphate (dNTP), 1 mM primers, 3% (v/v) DMSO, 0.5 M betaine, and 0.4 units Phusion High-Fidelity DNA polymerase (New England Biolabs)
