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Description
The biotinylation reagent sulpho-NHS-LC-biotin (Pierce, Rockford, IL) was dissolved in PBS and added to cells at 0.5 mg ml 1

This facilitated the identification and characterization of macrophage M1 and M2 phenotypes through flow cytometry analysis, enabling the determination of changes in macrophage polarization states within the arterial tissues

Furthermore, the invasive nature of current tissue-based assays limits their clinical utility

Studies have shown that CoQ10 levels are lower in ME/CFS patients than in healthy individuals [133,134,135]
