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The biotinylation reagent sulpho-NHS-LC-biotin (Pierce, Rockford, IL) was dissolved in PBS and added to cells at 0.5 mg ml 1

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This facilitated the identification and characterization of macrophage M1 and M2 phenotypes through flow cytometry analysis, enabling the determination of changes in macrophage polarization states within the arterial tissues

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Furthermore, the invasive nature of current tissue-based assays limits their clinical utility

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Studies have shown that CoQ10 levels are lower in ME/CFS patients than in healthy individuals [133,134,135]

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