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Although lean mass was still greater in the L-citrulline + GSH group at 8 weeks, the difference from placebo was no longer significant

Extracellular vesicles as drug delivery systems: why and how

USA 112 , E4111E4119 (2015)
3.5 Removal of GST affinity tag after protease cleavage in solution If the protein was digested in solution, the target protein can be purified further by re-chromatography on the glutathione Sepharose column to remove any un-cleaved fusion protein and the GST moiety
