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A 20 g aliquot of total RNA was electrophoresed at 70 mV for 23 h in a 1.6% agarose/formaldehyde gel, transferred to a positively charged nylon membrane and probed with a 32 Plabeled PIG3 probe obtained from the verified IMAGE clone 859359 (supplied by ResGen)
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doi: 10.1128/msphere.00040-25 48 GlazierV
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