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Total protein concentration was measured using a BCA assay kit (TIANGEN)
Its called Lactigo
The activation of ULK1 kinase complexes, which facilitates the binding of the autophagy-associated protein 8 ATG8 protein family (also known as LC3s and GABARAPs) to phosphatidylethanolamines or phosphatidylserines and anchors them to autophagic membranes, is what initiates autophagy[109]
