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& Ruiz-Pesini, E

Materials Use Milli-Q-purified water or equivalent for the preparation of all buffers 2.1 pGEX gene fusion construction pGEX vector (see Figure 2) A cDNA or PCR product containing the coding sequence for the protein of interest and appropriate adjacent restriction sites that are compatible with the selected pGEX vector Restriction enzymes, agarose, 1X TAE buffer, T4 DNA ligase Competent E

A decrease in the catalytic activity of such enzymes or their complete inactivation can dramatically affect the ability of cells to carry out vital anabolic processes and reduce the adaptive potential of cells

Dans ce cas, notre organisme a besoin dantioxydants provenant de sources externes