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It is difficult to remove trace amounts of copper ions from the amino acids mixtures used in cell culture media
Nevertheless, some oxidants including nitric oxide, peroxynitrite, and H 2 O 2 have been shown to act as second messengers, affecting a number of different signaling pathways through the oxidative modification of redox-regulated key proteins [100,101]
Notably, both in-vivo and ex-vivo data showed that GSNO strengthened the outer blood-retinal barrier during endophthalmitis
