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CPA digestion of albumin To initiate in vitro enzymatic cleavage by CPA, unfused albumin (WT or QMP) or albumin fused to GLP-1, FIX 9 , or ACE2 13 (15 M), in DPBS (pH 7.4) (Sigma-Aldrich), were incubated with 30 g/mL of human pancreatic CPA (Elastin Products Company, Inc.) for 5 h at 37 C on a shaker platform set to 450 RPM

Forty-eight hours after injection, animals were anesthetized and perfused intracardially with saline followed by cold 1% paraformaldehyde/1.25% glutaraldehyde

The authors suggested that, although the inclusion of non-polar steroids into the micelles decreases as the PEO hydrophilic chain increases, the number of micelles in equimolar amounts of surfactants increases and consequently the total amount of steroid per mole of surfactant is greater, hence the observed increase in solubilizing efficiency with increased hydrophilic chain length when molar concentrations are considered