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Immunoblots to detect 4-HNE-adducted proteins and Ucp1, Prdm16, Pgc1, Dio2, and Cidea Samples prepared with Laemmli sample buffer and were separated by 10% SDS-PAGE gel and transferred to PVDF membrane
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This peptide ligand is an integrin to v and 3 components, which plays a vital role in bone metastasis of cancer cells (Nemeth et al., 2003
