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4 online tool ( Real-time qRT-PCR Messenger RNA (mRNA) levels of ACC, PGC-1, PGC-1, C/EBP, FAS, SREBP-1c, rhodopsin, GFAP, RPE65, IGF-1, IGFBP3, and IGF-1R were analyzed by quantitative real-time RT-PCR (qPCR) using specific primer pairs (Table 1) using Primer3 software

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In addition to IGF-1 and IGF-1R, overexpression of IGF-2 and INSR-A is reported in a wide range of human cancers, including breast, colorectal, prostate, and lung cancers, and is also associated with a poor prognosis [2123]

P.et al (2005)