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Structural insights into the distal CTD were first obtained from a crystal structure of an isolated engineered domain derived from turkey PLC (Singer et al., 2002), revealing an unusual approximately 140 long helical bundle composed primarily of three long, kinked helical spans and several shorter bridging helices (Figs

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To confirm this important finding, membrane permeability was also monitored by following LacZdependent hydrolysis of the chromogenic substrate ONPG ( ortho nitrophenyl Dgalactopyranoside) in cells deficient for the uptake system LacY