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Samples were cleared by centrifugation at 4 C 10,000 rpm for 5 min, and the supernatants were analyzed and quantified with the SAM standard curve
Heating agarose fully dissolves it in the liquid and detangles all of the individual polysaccharides
In such a case, the ownership percentage of all existing shareholders, including founders and investors, would be diluted unless they choose to increase their shareholding by purchasing more shares
