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cDNA was diluted to 5 ng/l, and gene expression was measured with quantitative RT-PCR using the KAPA Sybr Fast kit (Roche, SFUKB) on a 384-well format Viia 7 machine (Applied Biosystems)

Though these physiotherapy methods are generally acceptable, using an animal treadmill would provide consistent and effective physiotherapy

Lonza Walkersville Inc., Walkersville, MD)

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