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Chen, Q., Li, Z., Pinho, R
I was also impressed that I didn't loose the luxury feel
After plasma membrane sheets were obtained and verified by microscopic observation, the ice-cold K-Glu buffer was replaced with room-temperature K-Glu buffer, and they were incubated with 2 M recombinant -SNAP WT or M105I for 5 min (PBS alone was used as negative control)
Elute the bound protein of interest with 5x bead volume of Elution Buffer
