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Description
Jack CR, et al
In patients with renal dysfunction, refractory hypotension, severe metabolic abnormalities, active seizure, or persistent coma, extracorporeal removal by haemodialysis or haemofiltration may be considered, although there are no controlled trials that demonstrate an improvement in outcome with these measures [8890]
C-terminally 6xHis-tagged HID was amplified using RF1396 and RF1397 and cloned into pACYC-Duet (MCS1) linearised using RF1398 and RF1400 using Gibson assembly, and SLP L or LID were amplified using RF1394 and RF1395 or RF1395 and RF1396, respectively, and cloned into MCS2 using Nde I- Kpn I restriction cloning
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