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Also in these colon organoids, no major morphological changes were observed in confocal images (Supplementary Fig
& Minh, B
The beads were washed twice with NP-40 Wash Buffer (20 mM HEPES/KOH pH 7.5, 150 mM KOAc, 5 mM MgCl 2 , 1 mM DTT, 0.1% IGEPAL CA-630) and once with Nikkol Buffer (20 mM HEPES/KOH pH 7.5, 150 mM KOAc, 5 mM MgCl 2 , 1 mM DTT, 0.05% octaethylene glycol monododecyl ether)
