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The lack of FASN also led to an inversion of the NAD + /NADH quotient, indicating a decreased capacity of NAD + regeneration (Supplementary Fig

In vitro -cultured microspores challenged by stress factors during ME seem to be a perfect model for investigation of GSH homeostasis in single cells and microspore-derived embryos

Apart from performing key roles in cellular redox homeostasis and the antioxidant defense, GSH is involved in the chelation and detoxification of free metal (reviewed by Anjum et al., 2012)

After electrophoresis, the proteins were transferred to nitrocellulose membranes using a semidry transblotter
