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Briefly, cDNA was synthesized using the Trancriptor One-Step RT-PCR (Roche) kit with a combination of specific primers (forward primer labeled 5Bo8254 and reverse primer 5Bo8707) yielding a final product of 454 base pair (bp) (nucleotide positions 8254-8707, H77 reference genome- GenBank accession number AF009606)
In contrast, Dehghani et al
