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As a consequence, the number of yearly new infections (1.4 million) exceeded the number of people receiving treatment (0.65 million) in 2020 2

f , Body temperature at indicated time points following transfer of mice from room temperature to cold (6 C) (n = 5 Control and n = 6 NDI1)

Superoxide reporters of the DHE family yield non-specific raw fluorescence, thus quantification must rely on chromatographic separation of DHE2-hydroxyethidium (2-OH-E) by HPLC to measure O 2 and discriminate it from ethidium or other adducts, ideally alongside SOD controls and pathway-selective perturbations (e.g., NOX2 inhibitors, mitochondrial site inhibitors) for source assignment

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