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Description
8 , e2934 (2017)

TB-500 is a synthetic peptide modeled after thymosin beta-4 and is frequently studied for its influence on cell migration, actin regulation, and muscle repair processes

To quantify the mScarlet fluorescence in individual chromatography fractions, 100 L samples were each transferred to a 96-well Nunc black microwell plate (Thermo Fisher Scientific) and the fluorescence intensities were measured in a Synergy 2 microtiter plate reader with 530/25 nm and 590/35 nm filters for excitation and emission, respectively

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