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The separation of disulfide and H 2 O 2 responses in these bacteria seems to support our conclusion that these phenomena can be unlinked

Lane 1: HEK-293 cell lysate (20 g/Lane) Lane 2: HepG2 cell lysate (20 g/Lane) Lane 3: SH-SY5Y cell lysate (20 g/Lane) Lane 4: HT-29 cell lysate (20 g/Lane) Lane 5: Human liver tissue lysate (40 g/Lane) Lane 6: Human kidney tissue lysate (40 g/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds

Glutathione (GSH) is a ubiquitous thiol tripeptide that protects against oxidative stress-induced damage by neutralizing reactive oxygen species (5)

Glutathione, Certified Reference Material (Sigma-Aldrich) RM628.00 Brand: Sigma-Aldrich Description CAS number: 70-18-8 Chemical Formula: H2NCH(CO2H)CH2CH2CONHCH(CH2SH)CONHCH2CO2H Molar Mass: 307.32g/mol Synonyms: -L-Glutamyl-L-cysteinyl-glycine, L-Glutathione reduced, GSH Amino Acid Sequence -Glu-Cys-Gly General description Glutathione (GSH) is the most important nonprotein thiol widely distributed in animal tissues, plants, and microorganisms