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7 days later, the post-treated cells were washed with PBS, immobilized with paraformaldehyde, and then stained with 10% crystalline violet

For more than a century, methylene blue (MB), a tricyclic phenothiazine often referred to as methylthionine hydrochloride, has been used for a variety of medical purposes, including targeting specific cellular targets

SQH also concentration-dependently inhibited Cyt c release into the cytoplasm and improved MMP (Figures 5C,D), underscoring its role in maintaining mitochondrial integrity through inhibiting lipid peroxidation in mitochondria