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[DOI] [PubMed] [Google Scholar] 138.Charuwichitratana S, Wattanakrai P, Tanrattanakorn S
Complementary single-stranded DNA oligonucleotides encoding the sgRNAs were synthesized (Sigma Aldrich Genosys) and annealed, then ligated into BbsI -digested PX459 vector
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