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The results indicated that the binding affinity of mutant TEV proteases toward the substrate was similar to that of the native protease, but the k cat of the new T17S/N68D/I77V+S219V mutant TEV protease was 50fold greater than that of the native TEV protease

doi: 10.1016/j.preteyeres.2011.08.001

doi: 10.21037/cdt-20-129