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Quantitative measurements of IGF-1, NKB, and GnRH were performed using commercial ELISA kits (Thermo Fisher, USA), strictly following the manufacturers protocols
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The closest FDA-approved IGF-1 product is mecasermin (Increlex) , native recombinant human IGF-1, approved only for the long-term treatment of growth failure in pediatric patients with severe primary IGF-1 deficiency or GH gene deletion with neutralizing antibodies
