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The supernatant (cytosolic proteins) was collected, and the pellet (membrane proteins) was resuspended in RIPA buffer with detergents and centrifuged at 16,000 g for 45 min at 4 C
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43 ) in the range found in mammalian cells 44
Quantification of Western blot of p-RyR2 Ser2808, p-TnI Ser23/24, and p-PLB Ser16/Thr17 normalized to the protein level of CSQ2, and then reported as a percentage of the highest value in the groups
