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Accordingly, oxythiamine treatment decreased cell proliferation in mutant but not WT chondrocytes (Extended Data Fig
Reverse transcription experiments were performed to obtain cDNA templates according to the instructions (Accurate Biology, Hunan), and further real-time quantitative PCR experiments were carried out to ascertain the expression of target genes using the QuantStudio Q7 RTPCR system
10.1371/journal.pone.0171904
