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8 weeks: 2 vials use 4 mL total

Mice were first familiarized to the treadmill for three non-consecutive days for 5 min at a walking speed (8 m/min)

2.2 Experimental design The Design Expert software (version 13, Stat-Ease Inc., Minneapolis, MN, United States) was employed to create a three-factor, three-level (3) Box-Behnken Design (BBD) with five center points, resulting in 17 experimental runs as depicted in Table 1

392,393,394 Recent findings suggest that H 2 S donor hold potential in the treatment of pancreatic cancer
