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This, together with the requirement of high stability of the purified protein in solution, hinders the application of this technique to membrane proteins and protein complexes

10.1016/j.bbrep.2023.101476 56 IncalzaM

It cannot confirm sterility

One of various possible hypotheses for the rapid increase in the incidence of such conditions is the damage to the intestinal and/or respiratory epithelial/ skin integrity observed in their course and the associated disruption of the epithelial barrier, causing it to leak and increase permeability
