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doi: 10.1074/jbc.RA117.000635
COVID-19 and the digestive system
His-tagged proteins and enzymes can be purified using immobilized-metal affinity chromatography (IMAC), which exploits the strong co-ordinate bonds formed the his-tag and the transition metal ions in the support (or stationary phase), using Ni columns to trap the desired protein 13,14,15,16
