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For example, patients receiving high-dose methotrexate require serial testing of urine pH and measurement of circulating methotrexate drug levels at regular intervals to guide leucovorin dosing and inform appropriate glucarpidase administration 9,25

Although it has a powerful physiological function, more studies have focused on its pathological role, mainly because it is involved in the pathogenesis of several neurodegenerative diseases, such as Parkinsons disease (PD) and dementia of Lewy body (DLB) [7, 8]