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Cells were sustained in Dulbeccos modified Eagle medium (DMEM) with pyruvate (Corning, MT-50-003-PC) supplemented 3.7 g l 1 sodium bicarbonate (Sigma, S6297), 10% heat-inactivated fetal bovine serum (FBS) (Gibco, 26140079 and Cytiva HyClone, SH3039603HI) and 1% penicillinstreptomycin solution (P/S) (Sigma, P4333)

Taking more than the recommended dose wont necessarily increase the benefits and could lead to unwanted side effects

Side Effects / Risks While Glutathione injections are generally safe, there may be some mild side effects, such as redness or swelling at the injection site

The effect of body mass index on sperm DNA fragmentation: a systematic review and meta-analysis