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Description
Western blot analysis CCA cells and tumor tissue were lysed with 1X RIPA reagent (Cell Signaling Technology, Danvers, MA, USA) and the protein concentration was measured with Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Inc)
doi: 10.3390/pharmaceutics15102444
The cleared protein antibody beads complex was incubated at 100 C for 10 min
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