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Freeze substitution was performed in a Leica EM AFS2 freeze substitution unit in dry acetone supplemented with 0.3% uranyl acetate at 85C for 16 h before gradually warming up to 50 C over a 5-h period

Elsewhere, such as at Gran Quivira, where there has been substantial modern and historic activity, presumed mobile group loci are also lacking

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When this occurs, the glucose transporter type 4 (GLUT4) is not transported into the cell surface and the glucose cannot be uptaken into the cell