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Wu L, Seung E, Xu L, et al

Folate (as L-5-MTHF) 400 mcg DFE

Bacteriostatic water has been a standard pharmacopoeial diluent since the 1940s, when benzyl alcohol was introduced as a multi-use preservative for parenteral preparations

Caspase-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)