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The lysate was subsequently centrifuged (12000 g, 4 C, 5 min), denatured, analysed by SDSPAGE, and transferred to PVDF membranes (IPVH15150, Millipore)
Copper peptides aren't creating new congestion

Additionally, the concentration of AOD9604 in the synovial fluid after injection was estimated to be about 0.19 mg/mL, similar to concentrations used in previous GH injection studies in animal models

k cat and K m
