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copper and glutathione Detection Characterization of a Conjugate of a Novel Complex glutathione copper Glutathione: subcellular distribution
Description
doi: 10.1615/critrevtherdrugcarriersyst.v24.i1.30
Sharma M, Khan FH, Mahmood R
To prepare the Transwell assay, murine melanoma cells (1 10 5 cells per insert) cultured in 200 l RPMI1640 medium supplemented with 0.5% FBS were plated on the upper insert (6.5 mm diameter transwell with 8 m pores, Corning), while 800 l RPMI1640 medium supplemented with 0.5% FBS, with or without hHGF or dimeric macrocycle was added to the bottom chamber
Feeling nothing at this dose is completely normal and expected
