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Tissue homogenization was performed with the Precellys Evolution Homogenizer (Bertin Technologies, France, P000062-PEVO0-A) at 5500 rpm for 3 cycles of 10s with a 10s pause, and tissues were lysed in TRIzol (Thermo Fisher, United States, 15596026) simultaneously

5-Amino-1-MQ iodide, 5-Amino-1-methylquinolinium, iodide (1:1), 5-Amino-1MQ iodide

20 Moreover, at 7 days, the Kyoto Encyclopedia of Genes and Genomes analysis of the proteomics data revealed that SPPEPS activated insulin signaling pathways through a gene crucial for cartilage development, namely GSK-3

Attach the blue needle, again without letting the tip of the syringe or the connecting part of the blue needle touch anything else Step 12