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Total RNA was isolated from the frozen biopsies using TRIzol reagent (Invitrogen, Carlsbad, USA) and purified with the RNeasy Mini Kit (Qiagen, Venlo, the Netherlands)

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One of the considered mechanisms of migraine pathogenesis concerns the involvement of tumor necrosis factor (TNF-), which results in the occurrence of migraine symptoms through initiation of neuronal hyper-excitability, stimulation of nociceptors, and prostanoid production which, in turn, leads to initiation of neuroinflammation [69]