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Methionine (Met) and cysteine, which contain sulphur, are probably the most susceptible to ROS oxidation ( MSR genes ( Additionally, not only central metabolism links to redox signalling in fruit but also more specialised pathways involving phytohormones ( Figure 4 )

The combined protein sample was placed in a 10-kDa cutoff dialysis cassette (66382, Thermo Fisher Scientific) pre-equilibrated with PBS buffer for 5 minutes

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GST-tagged hinT recombinant proteins were immobilized to Octet anti-GST biosensors (Sartorius 18-5096)