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Relative change is splicing was quantified using MAPT isoform values standardized to the average of GAPDH and HPRT1 Ct values, then the ratio of each isoform (1N/0N and 2N/0N) were calculated before normalizing to the average of the wild type samples

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A Samanta TET Hughes VY Moiseenkova-Bell 2018 Transient Receptor Potential (TRP) Channels Subcell Biochem 87:141-165 Sideris A, Doan LV